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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with <t>a</t> <t>UV‐ozone</t> treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.
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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with <t>a</t> <t>UV‐ozone</t> treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.
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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with <t>a</t> <t>UV‐ozone</t> treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.
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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with <t>a</t> <t>UV‐ozone</t> treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.
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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with <t>a</t> <t>UV‐ozone</t> treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.
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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with <t>a</t> <t>UV‐ozone</t> treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.
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Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with a UV‐ozone treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.

Journal: Chembiochem

Article Title: Silanes to Silatranes: Robust Functionalization for Single‐Molecule Force Spectroscopy

doi: 10.1002/cbic.202500853

Figure Lengend Snippet: Differing surface‐reaction behaviors of APTES and APS. (A) APTES can react with a UV‐ozone treated glass surface to form the desired surface layer, but can also form crosslinked aggregates that adhere to the cantilever and surface. This can prevent the SMFS study from addressing a single molecule and, in extreme cases, result in films large enough to be seen in visible‐light microscopy of the cantilever ( blue arrow ). (B) APS also reacts with UV‐ozone treated glass to form a surface layer but resists crosslinking in solution. This results in the desired single‐molecule tether.

Article Snippet: Briefly, surface functionalization began with UV‐ozone cleaning (PSD Pro Series Digital UV Ozone System, PSDP‐UVD8, Novascan Technologies Inc.) for 30 min.

Techniques: Light Microscopy